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Research Interests
Laboratory diagnosis of human
and animal mycoses: direct microscopy of biological samples; isolation and
identification of fungal agents from culture: yeasts, dermatophytes fungi and
other fungi isolated from other superficial, subcutaneous and systemic mycoses.
Isolation and identification of tropical pathogenic fungi. Antifungal in
vitro sensitivity testing. Cryptococcus neoformans antigen detection.
Molecular identification of pathogenic fungi.
Current Projects
Molecular diagnosis of vaginal candidosis and antifungal sensitivity evaluation.
Study of in vitro sensitivity to the antifungal drugs used in the treatment of these infections: fluconazole, itraconazole,
ketoconazole and voriconazole. Direct detection of yeasts in clinical samples
using different molecular techniques based on PCR.
Not presently funded.
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Sensitivity study of pathogenic yeasts isolated from blood stream ans CSF to
different compounds with antifungal activity. Gene-targeting with natural compounds to control pathogenic yeasts. Not presently funded.
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Prevalence and epidemiological studies of dermatophytes and yeasts in children
from Cova da Moura, a suburb slum of Lisbon.
Not presently funded.
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Molecular identification of yeasts directly from histopathologic preparations
from patients with invasive candidosis. Not presently funded.
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Development of a Point-of-Care (POC) DNA-based diagnostic kit for the direct
detection and highly specific identification of Cryptococcus neoformans. Not
presently funded.
Recent publications
Kim JH, Faria NC, Martins Mde L, Chan KL, Campbell BC. 2012. Enhancement of antimycotic activity of amphotericin B by
targeting the oxidative stress response of Candida and Cryptococcus with natural dihydroxybenzaldehydes. Frontiers
in Microbiology. 2012;3:261. Epub 2012 Jul 19.
Kim J.H., Chan K.L., Faria N.G, Martins M.L.and Campbell B.C. 2012. Targeting the oxidative stress response system of
fungi with safe, redox-potent chemosensitizing agents. Frontiers in Microbiology. 3:88. doi: 10.3389/fmicb.2012.00088.
Daniela Cunha, Cristina Amaro, María Raquel Vieira, María da Luz Martins, Ana Paula Maduro, João Inácio, Ana Afonso,
Gabriela Marques Pinto, Jorge Cardoso 2012. Phaeohyphomycosis caused by Alternaria infectoria presenting as multiple
vegetating lesions in a renal transplant patient. Rev Iberoam Micol. 29(1):44– 46.
Jong H Kim; Natália C Faria; Maria de Luz Martins; Kathleen L Chan; Bruce Campbell 2011. Increased antimycotic activity
of amphotericin B against clinical strains of Candida and Cryptococcus through chemosensitization by redox-active
dihydroxybenzaldehydes. Lett. Appl. Microbiol., May;52(5):506-1.
Teles F.R.R., Martins M.L. 2011. Laboratorial diagnosis of paracoccidioidomycosis and new insights for the future of
fungal diagnosis. Talanta 85: 2254– 2264.
Faria, N.C.G, Kim, J.H., Gonçalves, L.A.P., Martins, M. de L., Chan, K.L., Campbell, B. C. 2011. Enhanced activity
of antifungal drugs using natural phenolics against yeast strains of Candida and Cryptococcus. Lett.Appl.
Microbiol. 52(5): 506-513.
Bento D, Tavares R, Martins ML, Faria N, Maduro AP, Araújo C, Ventura F, and Mansinho K. 2010. Atypical presentation of
Entomophthoromycosis caused by Conidiobolus coronatus, Medical Mycology 48: 1099–1104.
Pfaller M.A., Diekema D.J., Gibbs D.L., Newell V.A., Ellis D., Tullio V., Rodloff A., Fu W., Ling T.A., and the Global
Antifungal Surveillance Group (includes Martins M.L. from IHMT). 2010. Results from the ARTEMIS DISK Global Antifungal
Surveillance Study, 1997 to 2007: a 10.5-Year Analysis of Susceptibilities of Candida species to Fluconazole and
Voriconazole as Determined by CLSI Standardized Disk Diffusion. J. Clin. Microbiol. 48: 1366–1377.
Lucas S, Martins ML, Flores O, Meyer W, Spencer-Martins I, Inácio J. 2010. Differentiation of Cryptococcus neoformans
varieties and Cryptococcus gattii using CAP59-based loop-mediated isothermal DNA amplification. Clin. Microbiol. Infect.
16(6): 711- 714.
Martins M. L., Ferreira A. S., Sampaio A., Vieira R. and Inácio J. 2010. Direct and specific identification of
Cryptococcus neoformans in biological samples using fluorescently labelled DNA probes, European Journal of Clinical
Microbiology & Infectious Diseases, 29(5): 571-576.
Santos M, Faria N, Iley J, Coles S, Hursthouse M, Martins ML, Moreira R. 2010. Facile synthesis and biological
evaluation of naphtha [2,3-d]isoxazole-4,9- diones as antifungal agents. Bioorganic & Medicinal Chemistry Letters 20: 193-195.
Pfaller M.A., Diekema D.J., Gibbs D.L., Newell V.A., Bijie H., Dzierzanowska D., Klimko N.N., Letscher-Bru V.,
Lisalova M., Muehlethaler K., Rennison C., Zaidi M., and the Global Antifungal Surveillance Group (includes
Martins M.L. from IHMT). 2009. Results from the ARTEMIS DISK Global Antifungal Surveillance Study, 1997 to 2007:
10.5-Year Analysis of Susceptibilities of Noncandidal Yeast Species to Fluconazole and Voriconazole Determined by
CLSI Standardized Disk Diffusion Testing. J. Clin. Microbiol. 47: 117-123.
Vieira R., Botelho M M, Lima C, Lázaro A, Martins M L. 2009. Intra-abdominal abscess - a clinical manifestation of
african histoplasmosis. Journal de Mycologie Médicale/Journal of Medical Mycology. 19(2): 134-137.
Pfaller MA, Boykena L., Hollis R.J., Kroeger J., Messer S.A., Tendolkar S., Diekema D.J. 2009. ARTEMIS DISK Global
Antifungal Surveillance Group (includes Martins ML from IHMT). Comparison of results of fluconazole and voriconazole
disk diffusion testing for Candida spp. with results from a central reference laboratory in the ARTEMIS DISK Global
Antifungal Surveillance Program. Diagnostic Microbiology and Infectious Disease 65: 27–34.
Pfaller MA, Diekema DJ, Gibbs DL, Newell VA, Ng KP, Colombo A, Finquelievich J, Barnes R, Wadula J. 2008. Global
Antifungal Surveillance Group (includes Martins ML from IHMT). Geographic and temporal trends in isolation and
antifungal susceptibility of Candida parapsilosis: a global assessment from the ARTEMIS DISK Antifungal Surveillance
Program, 2001 to 2005. J. Clin. Microbiol. 46(3): 842-849.
Pfaller MA, Diekema DJ, Gibbs DL, Newell VA, Nagy KP, Dobiasova S., Rinaldi M., Barton R., A. Veselov, and the
Global Antifungal Surveillance Group (includes Martins ML from IHMT). 2008. Candida krusei, a Multidrug-Resistant
Opportunistic Fungal Pathogen: Geographic and Temporal Trends from the ARTEMIS DISK Antifungal Surveillance Program,
2001 to 2005. J. Clin. Microbiol. 46: 515–521.
Seixas F, Luz-Martins M, Pinto ML, Travassos P, Miranda M., Pires MA. 2008. A case of Pulmonary Cryptococcosis in a
Free-Living Toad (Bufo bufo)". Journal of Wildlife Diseases 44(2): 460-463.
Pfaller M.A., Diekema D.J., Gibbs D.L., Newell V.A., Meis J.F., Gould I.M., Fu W., Colombo A.L., Rodriguez-Noriega E.,
and the Global Antifungal Surveillance Group (includes Martins M.L. from IHMT). 2007. Results from the ARTEMIS DISK
Global Antifungal Surveillance Study, 1997 to 2005: an 8.5-Year Analysis of Susceptibilities of Candida Species and
Other Yeast Species to Fluconazole and Voriconazole Determined by CLSI Standardized Disk Diffusion Testing J. Clin.
Microbiol. 45: 1735–1745.
Carvalho A., S. Oliveira, M.L. Martins, C. Pina-Vaz, A. Rodrigues, F. Rodrigues. 2007. Multiplex PCR identification of
eight clinically relevant Candida species. Med Mycol. 18:1-9.
Pfaller M.A., Diekema D.J., Colombo A.L., Kibbler C., Ng K.P., Gibbs D.L., Newell V.A., and the Global Antifungal
Surveillance Group (includes Martins M.L. from IHMT). 2006. Candida rugosa, an Emerging Fungal Pathogen with
Resistance to Azoles: Geographic and Temporal Trends from the ARTEMIS DISK Antifungal Surveillance Program. J.
Clin. Microbiol. 44: 3578–3582.
Roque HD, Vieira R, Rato S, Luz-Martins M. 2006. Development of specific primers for the rapid detection of M. audouinii
in clinical samples. J. Clin. Microbiol. 44(12): 4336-4341.
Pfaller M.A., Diekema D.J., Mendez M. , Kibbler C., Erzsebet P., Chang S.-C., Gibbs D.L.,Newell V.A. and the Global
Antifungal Surveillance Group (includes Martins M.L. from IHMT). 2006. Candida guilliermondii, an Opportunistic Fungal
Pathogen with Decreased Susceptibility to Fluconazole: Geographic and Temporal Trends from the ARTEMIS DISK Antifungal
Surveillance Program. J. Clin. Microbiol. 44: 3551–3556.
Martins, M.L. 2006. Importância das Micoses Humanas em Saúde Pública. e.Ciência. 074: 35.
Pfaller M.A., Diekema D.J., Rinaldi M.G., Barnes R., Hu B., Veselov A.V., Tiraboschi N., Nagy E., Gibbs D. L., and the
Global Antifungal Surveillance Group (includes Martins M.L. from IHMT). 2005. Results from the ARTEMIS DISK Global
Antifungal Surveillance Study: a 6.5-Year Analysis of Susceptibilities of Candida and Other Yeast Species to Fluconazole
and Voriconazole by Standardized Disk Diffusion Testing. J. Clin. Microbiol. 43: 5848-5859.
Vieira MR, Martins ML. 2004. Micoses Subcutâneas. Trab. Soc. Port. Dermatol. Venereol. 62(2): 137-167.
Martins, ML. 2002. Polimorfismos Genéticos e Diagnóstico Molecular de Cryptococcus neoformans. Tese de doutoramento.
IHMT, Universidade Nova de Lisboa.
Vieira, MR, Luz-Martins M, Afonso A, Rego F, Cardoso J. 1998. Cutaneous alternariosis. Rev. Iberoam. Micol. 15: 97-99.
Moraes MF, Lima F, Niza-Pinheiro M, Consciência G, Lopes J, Pacheco T, Oliveira F, Luz-Martins M, Mansinho K, Abecassis P.
1997. Combined medical and surgical therapy of disseminated African histoplasmosis with a successful outcome: two case
reports. Mediterranean Journal of Infecious and Parasitic Diseases 12: 115-124.
Chapters in books:
Filipe L., Maduro A.P., Teles F, Martins M.L. 2012. Specific detection of Aspergillus species in blood from patients with
invasive aspergilosis. In Microbes in Applied Research, Current Advances and Challenges. Ed. A. Mendez-Vilas, World
Scientific Publishing. pag. 567. ISBN 10981-4405-03-5.
Maduro A.P., Pereira A., Martins H.M., Dhieb C., Zouaqui N.S., Teles F., Martins M.L. 2012. Candida species isolated
from human infections from Tunisian and Portuguese patients: molecular identifications and in vitro antifungal
susceptibility. In Microbes in Applied Research, Current Advances and Challenges. Ed. A. Mendez-Vilas, World
Scientific Publishing. pag. 500. ISBN 10981-4405-03-5.
Martins M. L., Inácio J. 2011. Microscopic detection of specific fungi using fluorescence in situ hybridization.
In: O'Connor L., Glynn B. (Eds.) Fungal Diagnostics - Methods in Molecular Biology. Humana Press, Totowa, USA (Aceite)
Pereira F., Esteves A., Martins M.L., 2011. Diagnóstico Laboratorial em Micologia Médica, Capítulo 15 - Identificação
Cultural e Bioquímica. In: Meliço- Silvestre A., Barroso H., Taveira N. (eds) Microbiologia Médica. LIDEL, Lisboa
(em fase de provas finais)
Lança A., Almeida I., Martins H. M., Bernardo F., Guerra M., Inácio J., Martins M.L. 2010. Polyphasic characterization
of Aspergillus fumigatus strains causing infection in parrots and dolphins. In “Microorganisms in Industry and
Environment – From scientific and industrial research to consumer products”, Ed. A. Mendez-Vilas, pp. 515-517,
World Scientific Publishing Co. (ISBN-13: 978-981-4322-10-2)
Lança A., Almeida I., Martins H. M., Bernardo F., Guerra M., Inácio J., Martins M.L. 2010. An efficient molecular
typing assay for Alternaria spp. isolates. In “Microorganisms in Industry and Environment – From scientific and
industrial research to consumer products”, Ed. A. Mendez-Vilas, pp. 457-459, World Scientific Publishing Co. (ISBN-13: 978-981-4322-10-2)
Inácio J., Lucas S., Martins M. L., Flores O., Spencer-Martins I. 2007. Development of a Point-of-Care (POC) DNA-based
diagnostic kit for the direct detection and highly specific identification of Cryptococcus neoformans. Yeast 24: S66
(ISSN 0749 503X).
Inácio J., Lucas S., Martins M. L., Flores O., Spencer-Martins I. 2007. Identification of clinically relevant yeasts
combining a panfungal isothermal DNA amplification with hybridization to species-specific oligonucleotide probes.
Yeast 24: S66 (ISSN 0749 503X).
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