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Research Interests
Development of
molecular methods for the rapid identification of Gram-positive bacteria,
including staphylococci, mycobacteria and enterococci. Molecular
characterization of coagulase negative staphylococci.
Current Projects
As coordinator:
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Development of
molecular probes for the rapid diagnosis of staphylococci in clinical samples.
Fundação Calouste Gulbenkian, Ref. FCG 61056 (2003-2007).
As participant:
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Creation of a
Reference Culture Collection of antimicrobial resistant Gram-Positive bacteria
serving the national and international scientific and clinical communities.
Fundação Calouste Gulbenkian, Ref. FCG/61052 (2003-2006). PI: Hermínia de
Lencastre (ITQB/UNL).
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Efflux pumps
in Mycobacterium tuberculosis: molecular characterization of the efflux
mechanisms and the use of efflux inhibitors as new anti-TB drugs. Ref.
POCTI/SAU-MMO/59370/2004 (2005-2008). PI:
Miguel Viveiros
(IHMT/UNL).
Recent publications
Viveiros, M., M. Dupont, L.
Rodrigues, I. Couto, A. Davin-Regli,
M. Martins, J. M. Pagès, and L. Amaral. 2007. Antibiotic Stress, Genetic
Response and Altered Permeability of E.
coli. PLoS ONE. 2007 Apr 11;2:e365.
Martins, A., I. Couto, L. Aagaard, M. Martins, M. Viveiros, J. E. Kristiansen,
and L. Amaral. 2007. Prolonged exposure of methicillin-resistant Staphylococcus
aureus (MRSA) COL strain to increasing concentrations of oxacillin results
in a multidrug-resistant phenotype. Int. J. Antimicrob. Agents. 29:302-5.
Miragaia, M., J. C. Thomas, I.
Couto, M. C Enright MC, de Lencastre H. 2007. Inferring a population
structure for Staphylococcus epidermidis from multilocus sequence typing data. J.
Bacteriol. 189:2540-52.
Martins M., B. Santos, M., A.
Martins, M. Viveiros, I. Couto, A.
Cruz, The Management Committee Members of COST B16 of the European
Comission/European Science Foundation, J. M. Pagès, J. Molnar, S. Fanning, and
L. Amaral. 2006. “An instrument-free method for demonstration of efflux pump
activity of bactéria”. In vivo. 20:657-664.
Viveiros, M., C. Leandro, L. Rodrigues, J. Almeida, R.
Bettencourt, I. Couto, L. Carrilho,
J. Diogo, A. Fonseca; L. Lito, J. Lopes, T. Pacheco, M. Pessanha, J. Quirim, L.
Sancho, M. Salfinger, and Leonard Amaral. 2005. “Direct application of the
INNO-LiPA Rif. TB assay for the rapid identification of Mycobacterium
tuberculosis complex strains and detection of rifampin resistance in 360
smear positive respiratory specimens from an area of high incidence of
multidrug-resistant tuberculosis”. J. Clin. Microbiol. 43: 4880-4884.
Miragaia, M., I. Couto, and H. de Lencastre. 2005. Genetic diversity among
methicillin-resistant Staphylococcus
epidermidis (MRSE). Microb Drug Resist.11:83-93.
Carretto, E., Barbarini, D.,
Couto, I.,
De Vitis, D., Marone, P., Verhoef, J., De Lencastre, H. & Brisse, S. (2005).
Identification of coagulase-negative staphylococci other than Staphylococcus
epidermidis by automated ribotyping.
Clinical Microbiology and
Infection.
11:177-184.
Couto, I.,
S. W. Wu, A. Tomasz, and H. de Lencastre.
2003. “Development of methicillin resistance in clinical isolates
of Staphylococcus sciuri by transcriptional activation of the mecA homologue
native to the species”.
Journal of Bacteriology.
185: 645-653.
Miragaia, M., I. Couto, S. F. F. Pereira, J. Carriço, K. G. Kristinsson,
H. Westh, J. O. Jarløv, I. Santos Sanches & H. de Lencastre.
2002. "Molecular
characterization of methicillin resistant Staphylococcus epidermidis (MRSE)
clones: evidence of geographic dissemination". Journal of Clinical
Microbiology. 40:430-438.
Sá-Leão, R., I. Santos Sanches, I. Couto, C. R. Alves & H. de Lencastre.
2001. "Low prevalence of methicillin-resistant strains among
Staphylococcus aureus colonizing young and healthy members of the community in
Portugal".
Microbial Drug Resistance.
7: 237-245.
Couto, I.,
S. Pereira, M. Miragaia, I. Santos Sanches & H. de Lencastre.
2001. "Identification of human clinical
staphylococci by Internal Transcribed Spacer PCR (ITS-PCR)". Journal of
Clinical Microbiology. 39:3099-3103.
Santos-Sanches, I., R. Mato, H. de Lencastre, A.
Tomasz, CEM/NET Collaborators: S. Nunes, C.R. Alves, M. Miragaia, J. Carriço,
I. Couto, I. Bonfim, M.A. de Sousa, D. Oliveira, A. Gomes, M. Vaz, S.
Fernandes, S.C. Verde, A. Ávila, F. Antunes, R. Sá-Leão, J. Almeida, O. Melter,
M. Chung, International Collaborators: M.C. Brandileone, E. Castañeda, C.
Cocuzza, G. Echaniz-Aviles, I. Heitmann, M. Hortal, W. Hryniewicz, F. Jia, K.
Kikuchi, M. Konkoly-Thege, K.G. Kristinsson, J. Liñares, A. Rossi, E.Z. Savov,
J. Schindler, F. Solorzano-Santos, K. Totsuka, M. Venditti, P. Villari, H.
Westh, J.-S. Wu & R.C. Zanella. 2000. "Patterns of multidrug resistance among
methicillin-resistant hospital isolates of coagulase-positive and
coagulase-negative staphylococci collected in the International Multicenter
Study RESIST in 1997 and 1998".
Microbial Drug Resistance.
6:199-211.
Chung, M., H. de Lencastre, P. Matthews, A. Tomasz, & the Multilab Project
Collaborators: I Adamsson, M. Aires de Sousa, T. Camou, C. Cocuzza, A. Corso,
I. Couto, A. Dominguez, M. Gniadkowski, R. Goering, A. Gomes, K. Kikuchi, A.
Marchese, R. Mato, O. Melter, D. Oliveira, R. Palacio, R. Sá-Leão, I. Santos
Sanches, J.H. Song, P.T. Tassios, & Paolo Villari.
2000. "Molecular
typing of methicillin resistant Staphylococcus aureus by pulsed field gel
electrophoresis: Comparison of results obtained in a multilaboratory effort
using identical protocols and MRSA strains".
Microbial Drug Resistance.
6:189-198.
Couto, I.,
I. Santos Sanches, R. Sá-Leão & H. de Lencastre.
2000. "Molecular characterization of Staphylococcus sciuri
strains isolated from humans". Journal of Clinical Microbiology. 38:
1136-1143.
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