Degree in Applied Chemistry (spec.
Biotechnology), FCT/UNL, Portugal
PhD student at Free University of Amsterdam, the Netherlands
Post-doc at the Free University of Amsterdam, the Netherlands
Post-doc at CREM, FCT/UNL, Portugal
Assistant Professor, FCT/UNL, Portugal
We are currently interested in several topics in yeast biology that can be fruitfully explored using comparative genomics.
One of those topics concerns the molecular and functional characterization of mating systems in a group of fungi
(Pucciniomycotina, Basidiomycota) that includes the red (pigmented) yeasts, but also important plant pathogens like
the rust fungi. We recently uncovered a mating system in red yeasts, that we named pseudobipolar, which had never been
found before. As a result, we proposed a revised model for the evolution of mating systems in Basidiomycota and are
currently using Comparative Genomics to refine this model and trace its evolutionary history.
A second topic we are focusing on concerns the function and evolution of sugar transporters, presently with particular
emphasis on fructose transporters that play a major role in fructophily in yeasts. Fructophily is the preference for
fructose over glucose as carbon and energy source, a phenotype exhibited only by a very restricted number of species
among ascomycetous yeasts. We are particularly interested in the role of sugar transport in fructophily and are using
a combination of approaches including yeast physiology and genetics and comparative genomics to unravel the molecular
basis of fructophilic behavior. This knowledge will be valuable to devise strategies to correct incomplete fructose
fermentation, a problem often encountered during the production of alcoholic beverages from fruit juices, notably during
wine must fermentation.
Finally, we join efforts with the Evolutionary Ecology lab at CREM to address questions related to speciation in
Saccharomyces, aiming to understand the particularities of the extant distribution of the different species in
Nature and the ecological relevance of phenotypic differences between species.
Molecular and functional characterization of mating systems
José Paulo Sampaio (PI Evolutionary Ecology, CREM)
Function and evolution of sugar transporters
Marco Coelho (Post doc)
Márcia Palma (PhD student)
Madalena Oom (Senior Researcher, 20%)
Helena Sousa (Senior Researcher, 20%)
Carla Gonçalves (BI holder)
“Improvement of fructose fermentation by industrial strains of Saccharomyces cerevisiae”
“Comparative genomics approach to unravelling a new sexual reproduction system in fungi”
Coelho M.A., Gonçalves P., Sampaio J.P. (2011). Evidence for maintenance of sex determinants but not of sexual stages in red yeasts, a group of early diverged basidiomycetes. BMC Evol Biol. (2011) 11: 249.
Libkind D., Hittinger C.T., Valério E., Gonçalves C., Dover J., Johnston M., Gonçalves P., Sampaio J.P. (2011). Microbe domestication and the identification of the wild genetic stock of lager-brewing yeast. Proc Natl Acad Sci U S A. 108(35): 14539-44.
Gonçalves P., Valério E., Correia C., de Almeida J.M., Sampaio J.P. (2011). Evidence for divergent evolution of growth temperature preference in sympatric Saccharomyces species. PLoS One.;6(6): e20739.
Salema-Oom M., De Sousa H.R., Assunção M., Gonçalves P., Spencer-Martins I.( 2011). Derepression of a baker's yeast strain for maltose utilization is associated with severe deregulation of HXT gene expression. J Appl Microbiol. 110(1): 364-74.
Galeote V., Novo M., Salema-Oom M., Brion C., Valério E., Gonçalves P., Dequin S.( 2010). FSY1, a horizontally transferred gene in the Saccharomyces cerevisiae EC1118 wine yeast strain, encodes a high-affinity fructose/H+ symporter. Microbiology.156: 3754-61.
Coelho M.A., Sampaio J.P., Gonçalves P. (2010). A deviation from the bipolar-tetrapolar mating paradigm in an early diverged basidiomycete. PLoS Genet. 6(8).
Hittinger C.T., Gonçalves P., Sampaio J.P., Dover J., Johnston M., Rokas A. (2010). Remarkably ancient balanced polymorphisms in a multi-locus gene network. Nature. 464(7285): 54-8.
Leandro MJ, Fonseca C, Gonçalves P. (2009) Hexose and pentose transport in ascomycetous yeasts: an overview. FEMS Yeast Res. 9, 511-525.
Leandro M.J., Spencer-Martins I., Gonçalves P.(2008). The expression in Saccharomyces cerevisiae of a glucose/xylose symporter from Candida intermedia is affected by the presence of a glucose/xylose facilitator. Microbiology, 154:1646-1655.
Coelho M.A., Rosa A., Rodrigues N., Fonseca A., Gonçalves P. (2008). Identification of mating type genes in the bipolar basidiomycetous yeast Rhodosporidium toruloides: first insight into the MAT locus structure of the Sporidiobolales. Eukaryot Cell., 71053-71061.
Sampaio J.P., Gonçalves P.(2008). Natural populations of Saccharomyces kudriavzevii in Portugal are associated with oak bark and are sympatric with S. cerevisiae and S. paradoxus. Appl Environ Microbiol., 74:2144-52.
Leandro M.J., Goncalves P., Spencer-Martins I.(2006). Two glucose/xylose transporter genes from the yeast Candida intermedia: first molecular characterization of a yeast xylose/H + symporter. Biochem J., 395:543-549.